摘要：SummaryProteins of all living cells undergo a myriad of post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative species of African trypanosomes,Trypanosoma bruceiandTrypanosoma evansi. Ten PTM types with approximately 40,000 modified sites and 150 histone marks with a fine map on each protein of the two African trypanosomes were accomplished. The two biologically different trypanosomal species displayed distinct PTM-omic features, regulation pathways, and networks. Modifications in the proteins involved in the redox system were mainly upregulated inT. brucei, whereas proteins associated with motility were predominantly modified inT. evansi. The establishment of a database of multiple PTMs in the two parasites provides us with a deep insight into the biological mechanisms that underpin life processes in trypanosomes with different life cycles.Graphical AbstractDisplay OmittedHighlights•The first multi-proteomic profiles ofT. bruceiandT. evansiwere generated•Histones are modified heavily and are highly conserved in trypanosomes•The two biologically different trypanosomes displayed distinct PTM-omic features•Crosstalk between different PTMs involved in critical biological processesProteomics; Metabolomics
