标题：Comparative Analysis of Leaf Trichomes, Epidermal Wax And Defense Enzymes Activities in Response to Puccinia horiana in Chrysanthemum and Ajania Species
摘要：AbstractWhite rust caused byPuccinia horianais a destructive disease of chrysanthemum plants. To better understand the resistance mechanisms of composite species to this disease, the leaf cuticular traits, antioxidant and defensive enzymes activities of immune (Chrysanthemum makinoivar.wakasaense) and highly susceptible (Ajania shiwogikuvar.kinokuniense) species were compared. Trichome density of two species was markedly different, negatively associated with plant resistance toP. horiana. Total wax load inC. makinoivar.wakasaensewas two times more than that inA. shiwogikuvar.kinokuniense. The wax composition in immune one was abundant in esters and primary alcohols. Superoxide dismutase (SOD, EC 1.15.1.1), peroxidase (POD, EC 1.11.1.7), polyphenoloxidase (PPO, EC 1.14.18.1 or EC 1.10.3.2) and phenylalanine ammonia lyase (PAL, EC 4.3.1.5) activities were investigated. InC. makinoivar.wakasaense, the activity of SOD and POD increased rapidly after inoculation, which might be non-host induced reactive oxygen species (ROS) activated antioxidant enzymes, however SOD and POD remained a low and steady level in the highly susceptible one after inoculation. Quick increase in PPO activities after inoculation was observed in both species, however it remained higher inC. makinoivar.wakasaenseat the late period of inoculation. PAL inC. makinoivar.wakasaensewas induced after pathogen inoculation, but not inA. shiwogikuvar.kinokuniense, suggesting that these two enzymes might contribute to the resistance toP. horiana.
