摘要：The goal of this paper is to demonstrate in vitro techniques of culturing Entomopathogenic nematode and the diet on which EPN survive and flourish. The in vitro production of nematodes is essential for the commercial use of EPN. The competence of a range of media to promote growth and development of the nematodes has been evaluated. The diet consists of a base diet plus a nutritive supplement. Nematode yield is a crucial factor for successful large-scale viable production of entomopathogenic nematode. Few attempts at in vitro culture of EPNs have been undertaken. The various methods used to commence cultures are described. The diet named Kabuli gram medium constitute Nutrient broth (0.88g), yeast extract (0.32g), Kabuli gram (16g), soybean oil (10.40ml), and distilled water (60ml) proved effective, very nutritious and appropriate for nematode culture, with this diet EPN flourish in good quantity. In vitro culturing requires more attention, nominal temperature. A small negligence will destroy the colonies of EPN in no time. However, quality and quantity of in vitro produced Entomopathogenic nematode vary considerably, depending on diet and temperature. The EPN cultured, were used for the biocontrol of Helicoverpa armigera in further study on the crops of Pisum sativum and Cicer arietenum. The production and culture of EPN had help in the biocontrol. EPN used in present and further study were Steinernema masoodi and Oscheius nadarajni.
