摘要：Expressed sequence tag SSRs (EST-SSRs) are gene-derived SSRs that are easily built up using the tremendous ever-increasing cDNA clones and EST databases. Two hundred thirty eight SSR- containing EST were recognized after mining 8430 F icus carica EST tandems that correspond approximately to 4873 Kb. A total of 360 EST-SSRs were recognized and 39 newly developed primers were designed. BLASTX identified the function of 153 (64%) EST sequences. Genetic diversity among the seven fig accessions were performed using the 22 amplified EST-SSRs. The generated dendrogram using UPGMA clustering was successful in distinguishing various fig accessions with a sim ilarity coefficients ranged from a high of 0.93 to a low of 0.72. The present investigation is the first direct attempt to build up a large number of EST-SSR markers in F icus carica. These EST-SSRs will not only increase the SSRs lim ited availability in Ficus carica but also they can be used across related species.
关键词：BLASTX;Fig;Functional SSRs;Genetic diversity;Gene- based SSRs;Microsatellite markers
