期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2018
卷号：15
期号：8
页码：561-568
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Background: Ziziphus genus (jujube) is a fruit, which is commonly found and used in Eastern traditional medicine for various conditions. Immunomodulation and anti-oxidant activities of jujube fruit extract are reported. This study aimed to observe immunomodulation effects of green (unripe) and ripe jujube fruit extract on peripheral blood mononuclear cell (PBMC) proliferation by carboxyfluorescein diacetate (CFSE) fluorescence, cytokine profiling by enzyme-linked immunosorbent assay (ELISA), and intracellular H2O2 level by dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence. Results: Treatments of PBMC with both green and ripe pulp extracts resulted in non-significant increases, about 1.01 - 1.15 times, in PBMC proliferation compared to the untreated group (p > 0.05). The pro-inflammatory cytokine (IL-1β) and regulatory Th2 cytokine (IL-10) levels were significantly higher in PBMC treated with ripe jujube fruit extract than in the cells stimulated with green jujube extract (p 2O2 reduction was observed in PBMC treated with the green pulp extract compared to the ripe pulp extract (p Conclusions: This study provides practical information about an effect of jujube staging on some cytokine inductions. The reduction of intracellular oxidative stress seems to involve these cytokine induction phenomena.
