期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2020
卷号：17
期号：2
页码：96-102
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Attachment ability of bifidobacteria strains to the human intestinal surface is an important criterion as a probiotic candidate. However, attachment activity is influenced by external and internal conditions. This study was conducted to screen cell surface hydrophobicity and adhesion scores of bifidobacteria strains. Attachment conditions (pH and exposure time) of selected strains to human enterocyte-like Caco-2 cell lines were subsequently investigated. Three different solvents (n-hexadecane, Toluene, and Xylene) were used in cell surface hydrophobicity analysis. Based on the results obtained, xylene presented consistent cell hydrophobicity activity in all strains used. Bifidobacterium pseudocatenulatum KAKii (wild type strain) gave promising cell hydrophobicity activity with no significant difference (p > 0.05) when compared to Lactobacillus plantarum NBRC 3070 with xylene as a solvent, and also presented a significantly higher attachment score (p B. pseudocatenulatum KAKii to Caco-2 cells revealed that this strain was favored to attach to the intestinal cell line at pH 6 and after 120 min of exposure. Further optimization of attachment conditions will be carried out.
关键词：Bifidobacterium strains;cell-surface hydrophobicity;pH conditions;time of exposure;Caco-2 cell lines
