期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2020
卷号：17
期号：3
页码：285-292
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Salt stress affects crop productivity by altering the biology of the plant and limiting productivity. Hordeum vulgare is the most tolerant cereal crop, with rich genetic resources. The underlying molecular mechanism involved in salt stress response is yet to be comprehensively addressed. A total of 305 proteins are involved in the network. We attempted to find relationships between a few representative stress-responsive proteins of osmotic (pip1), ionic (K+/Na+ ratio in the leaf sheath, HvHAK, HAK4, NHX1 and Ha1), and oxidative stress (APX, CAT1, SOD1) from the public protein database to identify the most influential protein in the network. Further, the salt response proteins were analyzed for their enriched protein domains, Kyoto Encyclopedia of Genes and Genomes pathways, molecular functions, and cell localization. The graph theory analysis of the large data could provide clues for the identification of potential biomarkers for salt stress in barley. An experiment was performed in three accessions of H. vulgare to identify the reliability of the theoretical network relationship in biological systems. The expression of the above-mentioned proteins was further experimentally proven based on the expression and assay.
