摘要：SummaryThe transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II. By using biochemical cellular fractionation and immunofluorescence analyses we reveal a previously uncharacterized nucleolar localization for BMAL1. We used an unbiased approach to determine the BMAL1 interactome by mass spectrometry and identified NOP58 as a prominent nucleolar interactor. NOP58, a core component of the box C/D small nucleolar ribonucleoprotein complex, associates with Snord118 to control specific pre-ribosomal RNA (pre-rRNA) processing steps. These results suggest a non-canonical role of BMAL1 in ribosomal RNA regulation. Indeed, we show that BMAL1 controls NOP58-associated Snord118 nucleolar levels and cleavage of unique pre-rRNA intermediates. Our findings identify an unsuspected function of BMAL1 in the nucleolus that appears distinct from its canonical role in the circadian clock system.Graphical AbstractDisplay OmittedHighlights•BMAL1 displays a circadian-independent localization in the nucleolus•Bmal1-deficient cells show altered nucleolar morphology•Interactome proteomics reveals that BMAL1 associates with nucleolar proteins•BMAL1 appears to play a non-canonical, non-circadian role in pre-rRNA processingCircadian Clock; Nucleolus; Ribosomal RNA; Molecular Mechanism of Gene Regulation
