摘要：SummaryComplex interactions between mRNAs and microRNAs influence cellular functions. The mRNA-microRNA interactions also determine the post-transcriptional availability of mRNAs and unbound microRNAs. MicroRNAs binds to one or more microRNA response elements (MREs) located on the 3′UTR of mRNAs. In this study, we leveraged MREs and their frequencies in cancer and matched normal tissues to obtain insights into disease-specific interactions between mRNAs and microRNAs. We developed a bioinformatics method “ReMIx” that utilizes RNA sequencing (RNA-Seq) data to quantify MRE frequencies across the transcriptome. We applied ReMIx to triple-negative (TN) breast cancer tumor-normal adjacent pairs and identified MREs specific to TN tumors. ReMIx identified candidate mRNAs and microRNAs in the MAPK signaling cascade. Further analysis of MAPK gene regulatory networks revealed microRNA partners that influence and modulate MAPK signaling. In conclusion, we demonstrate a novel method of using MREs in the identification of functionally relevant mRNA-microRNA interactions in TN breast cancer.Graphical AbstractDisplay OmittedHighlights•Bioinformatics method ReMIx identify differential microRNA response rlements (MRE)•Tumor-specific MREs frequency observed in triple-negative breast cancer (TNBC)•MRE analysis identify MAPK signaling genes as therapeutic target for TNBC•MREs frequency can be used to identify pathologically relevant pathwaysBiological Sciences; Bioinformatics; Cancer Systems Biology; Cancer
