摘要：SummaryHistone chaperone FACT is commonly expressed and essential for the viability of transformed but not normal cells, and its expression levels correlate with poor prognosis in patients with cancer. FACT binds several components of nucleosomes and has been viewed as a factor destabilizing nucleosomes to facilitate RNA polymerase passage. To connect FACT's role in transcription with the viability of tumor cells, we analyzed genome-wide FACT binding to chromatin in conjunction with transcription in mouse and human cells with different degrees of FACT dependence. Genomic distribution and density of FACT correlated with the intensity of transcription. However, FACT knockout or knockdown was unexpectedly accompanied by the elevation, rather than suppression, of transcription and with the destabilization of chromatin in transformed, but not normal cells. These data suggest that FACT stabilizes and reassembles nucleosomes disturbed by transcription. This function is vital for tumor cells because malignant transformation is accompanied by chromatin destabilization.Graphical AbstractDisplay OmittedHighlights•FACT is essential for viability of the tumor, but not for normal cells•FACT level depends on transcription, but transcription does not depend on FACT•FACT preserves nucleosomes during transcription to maintain chromatin integrity•FACT maintains chromatin in destabilized state during malignant transformationBiological Sciences; Molecular Biology; Chromosome Organization; Cancer
