摘要：SummaryHaploidization is invaluable for basic genetic research and crop breeding. The haploid bio-induction principle is an important topic that remains largely unexplored. In this study, both CenH3 RNAi andin vitroinhibition were used to simulate and induce haploids in allopolyploid crop. Notably,in vitroCenH3 inhibition showed that the results were much the same to that of RNAi in phenotype, chromosome behavior, microspore production, and haploid induction. Cytological analyses of RNAi and inhibitor-treated progenies revealed elimination of chromosomes, defective microspores with empty nuclei, thereby giving rise to pseudo male gametes, and haploid parthenogenesis induction. We found distinct defective empty microspores that were positively correlated with the decrease ofCenH3during RNAi manipulation. Investigation through bothin vivoandin vitrostudies revealed that haploidization was induced through the pseudo male gamete-mediated mock sexual reproduction. The present results provide insights for the haploid parthenogenesis induction process.Graphical AbstractDisplay OmittedHighlights•CenH3RNAi andin vitroinhibition can similarly induce haploids•We provide the cytological basis underlying parthenogenesis induction and haploids production•We report pseudo male gamete-mediated mock sexual reproduction during haploid parthenogenesis•We provide insights for the haploid parthenogenesis induction processBiological Sciences; Plant Biology; Plant Cytology
