摘要：SummarySomatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) inArabidopsis thalianaroots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960). The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes. The ATHB25/REM7 induced the expression of genes controlling shoot stem characteristics by ectopic expression in roots. Intriguingly, the restoration of root growth was seen in the consecutive and adjacent parts of the SSOs under gene induction conditions. Our findings thus provide insights into the development and regeneration of plant shoot stems.Graphical AbstractDisplay OmittedHighlights•Co-induction of ATHB25 and REM7 produces shoot stem-like organs (SSOs) in roots•SSOs exhibit negative gravitropism and differentiated vascular bundles•Shoot- and root-specific genes are up- and down-regulated, respectively, in SSOs•The restoration of normal root growth follows the SSO formationPlant Biology; Plant Genetics; Plant Development
