摘要：SummarySemi-artificial photosynthesis (biohybrid) provides an intriguing opportunity for efficient CO2-to-CH4conversion. However, creating a desirable semiconductor in biohybrids remains a great challenge. Here, by doping Ni into CdS nanoparticles, we have successfully developed theMethanosarcina barkeri-Ni:CdS biohybrids. The CH4yield by theM. barkeri-Ni(0.75%):CdS biohybrids was approximately 250% higher than that by theM. barkeri-CdS biohybrids. The suitable Ni dopants serve as an effective electron sink, which accelerates the photoelectron transfer in biohybrids. In addition, Ni doping changes the metabolic status ofM. barkeriand results in a higher expression of a series of proteins for electron transfer, energy conversion, and CO2fixation. These increased proteins can promote the photoelectron capture byM. barkeriand injection into cells, which trigger a higher intracellular reduction potential to drive the reduction of CO2to CH4. Our discovery will offer a promising strategy for the optimization of biohybrids in the solar-to-chemical conversion.Graphical AbstractDisplay OmittedHighlights•M. barkeri-Ni:CdS biohybrids were successfully developed for CO2reduction•A highestQEof 2.08% was achieved by theM. barkeri-Ni(0.75%):CdS biohybrids•Ni dopants effectively suppressed the electron-hole recombination in biohybrids•Ni doping changed the metabolic status ofM. barkeriin biohybridsNanoparticles; Biochemistry; Energy Resources
