摘要：SummaryIntegration of disease diagnosis and therapyin vivoby nanotechnology is a challenge in the design of multifunctional nanocarriers. Herein, we report an intelligent and degradable nanoreactor, an assembly of the 4-mercaptobenzonitrile-decorated silver nanoparticles (AgNPs@MBN) and the glucose oxidase (GOx)-loaded metal-organic-framework (ZIF-8@GOx), which can be activated by tumor microenvironment to start the catalytic cascade-enhanced chemo-starvation synergistic therapy and simultaneous self-sense of cellular glucose level. Under the mild acidic microenvironment of tumor, the nanoreactor will collapse to release GOx that triggers a catalytic cascade reactionin vivo, depleting glucose, etching AgNPs@MBN, and producing toxic H2O2, Ag+, and Zn2+ions, all of which work together to inhibit tumor growth. The AgNPs@MBN as SERS nanoprobe reads out glucose concentration noninvasively in tumor to achieve instant feedback of therapeutic progression. This work proposes a promising example of using enzyme-encapsulated biomineralized MOFs as an effective anticarcinogen for clinical applications.Graphical AbstractDisplay OmittedHighlights•This nanoreactor integratesin vivosensing and synergistic therapy capabilities•The ZIF-8 nanocarrier protects GOx from deactivation and immune clearance•The nanoreactor is biodegradable, avoiding the side effects on tumor-bearing mice•Instant non-invasive glucose feedback capability realized byin vivoSERSBiomaterials; Biomedical Engineering; Nanostructure
