摘要：SummaryButenyl-spinosyn produced bySaccharopolyspora pogonaexhibits strong insecticidal activity and a broad pesticidal spectrum. Currently, important functional genes involved in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficient understanding of its regulatory mechanism and improving its production by metabolic engineering. Here, we present data supporting a role of the SenX3-RegX3 system in regulating the butenyl-spinosyn biosynthesis. EMSAs and qRT-PCR demonstrated that RegX3 positively controls butenyl-spinosyn production in an indirect way. Integrated proteomic and metabolomic analysis,regX3deletion not only strengthens the basal metabolic ability ofS. pogonain the mid-growth phase but also promotes the flow of the acetyl-CoA produced via key metabolic pathways into the TCA cycle rather than the butenyl-spinosyn biosynthetic pathway, which ultimately leads to continued growth but reduced butenyl-spinosyn production. The strategy demonstrated here may be valuable for revealing the regulatory role of the SenX3-RegX3 system in the biosynthesis of other natural products.Graphical AbstractDisplay OmittedHighlights•Butenyl-spinosyn biosynthesis is highly sensitive to Pi control•RegX3 regulates polyP accumulation inS. pogona•RegX3 may indirectly regulate butenyl-spinosyn production•RegX3 plays an important role in the normal growth development ofS. pogonaGenetics; Microbiology
