摘要：SummaryPatient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating immune cells. Finally, we illustrate diverse downstream applications of this technique includingin vitrohigh-throughput chemotherapeutic screens,in situimmune cell characterization, andin vivopatient-derived xenografts. Thus, routine clinical FNA-based collection techniques represent an unappreciated substantial source of material that can be exploited to generate tumor organoids from a variety of tumor types for both discovery and clinical applications.Graphical AbstractDisplay OmittedHighlights•Fine-needle aspiration (FNA) is safe, minimally invasive, and widely used clinically•FNA is a source of material for organoid culture and personalized medicine•This technique requires minimal processing, preserving histology, and immune cells•Downstream applications: high-throughput screens, immune analysis, and xenograftsClinical Medicine; Tissue Engineering; Cancer
