摘要：AbsractAnthocyanins are the most widely produced secondary metabolites in plants, and they play an important role in plant growth and reproduction. The nitrogen source is an important factor affecting anthocyanin production, but the nitrogen concentrations on metabolism and the underlying genetic basis remain unclear. In this study,in vitroanthocyanin induction was conducted onMalus spectabilis. The leaf explants were cultivated in media containing different nitrogen concentrations. The results suggested that when the nitrogen contents decreased in limit, the color of leaf explants turned from green to red, and increased anthocyanin accumulation led to a change in phenotype. Furthermore, the content of other flavonoids, such as dihydroquercetin, epicatechin, and catechin, increased under low nitrogen conditions. The transcription levels of the general flavonoid pathway genes, from phenylalanine ammonia lyase (PAL) to anthocyanidin synthase (ANS), were associated with the concentration of corresponding flavonoid compounds and phenotype changes. In particular, the expression level ofANSincreased substantially under a low nitrogen treatment, which was significantly and positively correlated with the anthocyanin levels (R2 = 0.72,P< 0.05). The increased expression patterns of anthocyanin pathway genes were similar to that of the transcription factorMYB10. We further verifiedMYB10played an important role in the anthocyanin pathway in leaves ofMalus spectabilis. These results suggested that we can improve the desirable ornamental plant phenotypes by controlling nitrogen content. This process may offer clues to further development of new agricultural practices.
