摘要：SummaryBacteriophage phiYY is currently the only double-stranded RNA (dsRNA) phage that infectsPseudomonas aeruginosaand is a potential candidate for phage therapy. Here we applied RNA-seq to investigate the lytic cycle of phiYY infectingP. aeruginosastrain PAO1r. About 12.45% (651/5,229) of the host genes were determined to be differentially expressed genes. Moreover, oxidative stress response geneskatBandahpBare upregulated 64- to 128-fold after phage infection, and the single deletion of each gene blocked phiYY infection, indicating that phiYY is extremely sensitive to oxidative stress. On the contrary, another upregulated genePA0800might constrain phage infection, because the deletion ofPA0800resulted in a 3.5-fold increase of the efficiency of plating. Our study highlights a complicated dsRNA phage-phage global interaction and raises new questions toward the host defense mechanisms against dsRNA phage and dsRNA phage-encoded hijacking mechanisms.Graphical AbstractDisplay OmittedHighlights•The infection of dsRNA bacteriophage has a modest impact on host gene expression•Several differentially expressed host genes are essential for dsRNA phage life cycle•Anti-oxidative genes are highly expressed to support dsRNA phage infectionBiological Sciences; Genetics; Molecular Biology
