标题：The Importance of Earth Reference Controls in Spaceflight -Omics Research: Characterization of Nucleolin Mutants from the Seedling Growth Experiments
摘要：SummaryUnderstanding plant adaptive responses to the space environment is a requisite for enabling space farming. Spaceflight produces deleterious effects on plant cells, particularly affecting ribosome biogenesis, a complex stress-sensitive process coordinated with cell division and differentiation, known to be activated by red light. Here, in a series of ground studies, we have used mutants from the twoArabidopsisnucleolin genes (NUC1andNUC2,nucleolar regulators of ribosome biogenesis) to better understand their role in adaptive response mechanisms to stress on Earth. Thus, we show that nucleolin stress-related geneNUC2can compensate for the environmental stress provided by darkness innuc1plants, whereasnuc2plants are not able to provide a complete response to red light. These ground control findings, as part of the ESA/NASA Seedling Growth spaceflight experiments, will determine the basis for the identification of genetic backgrounds enabling an adaptive advantage for plants in future space experiments.Graphical AbstractDisplay OmittedHighlights•Ribosome synthesis is a target of spaceflight stressor effects on plant development•Nucleolin mutants promote a differential response to light/darkness stress•Red light and NUC2 may counteract the spaceflight alterations in gene expression•Ground controls are important for the interpretation of spaceflight -omics experimentsPlant Biology; Omics; Space Sciences
