摘要：SummaryResin embedding is widely used to dissect the fine structure of bio-tissue with electron and optical microscopy. However, it is difficult to embed large-volume tissues with resin. Here, we modified the formula of LR-White resin to prevent the sample cracking during polymerization process and applied this method to the intact brains of mouse, ferret, and macaque. Meanwhile, we increased the fluorescence preservation rate for green fluorescent protein (GFP) from 73 ± 4.0% to 126 ± 3.0% and tdTomato from 60 ± 3.3% to 117 ± 2.8%. Combined with the whole-brain imaging system, we acquired the cytoarchitectonic information and the circuit information such as individual axon and boutons which were labeled with multiple fluorescent proteins. This method shows great potential in the study of continuous fine microstructure information in large-volume tissues from different species, which can facilitate the neuroscience research and help the understanding of the structure-function relationship in complex bio-tissues.Graphical AbstractDisplay OmittedHighlights•Modified LR-White resin embedding was proposed to embed large-volume tissues•Retarder α-methyl-styrene was added to prevent cracking during polymerization•Resin formula was modified to preserve multiple fluorescent proteins•Microstructure information was acquired from the brains of different speciesTechniques in Neuroscience; Tissue Engineering; Biomedical Materials
