摘要：SummaryThe lipid bilayer plays a pivotal role in force transmission to many mechanically-gated channels. We developed the technology to monitor membrane diffusivity in order to test the hypothesis positing that Ca2+regulates open probability (Po) of cochlear hair cell mechanotransduction (MET) channels via the plasma membrane. The stereociliary membrane was more diffusive (9x) than the basolateral membrane. Elevating intracellular Ca2+buffering or lowering extracellular Ca2+reduced stereociliary diffusivity and increased METPo. In contrast, prolonged depolarization increased stereociliary diffusivity and reduced METPo. No comparable effects were noted for soma measurements. Although MET channels are located in the shorter stereocilia rows, both rows had similar baseline diffusivity and showed similar responses to Ca2+manipulations and MET channel blocks, suggesting that diffusivity is independent of MET. Together, these data suggest that the stereociliary membrane is a component of a calcium-modulated viscoelastic-like element regulating hair cell mechanotransduction.Graphical AbstractDisplay OmittedHighlights•Stereocilia membranes are highly diffusive as compared to the cell body membrane•Stereocilia membrane diffusivity varies with internal and external calcium levels•MET channel resting open probability increases as membrane diffusivity decreasesCellular Neuroscience; Sensory Neuroscience
