摘要：SummaryThe production of neurons from neural stem cells (NSCs) persists throughout life in the mouse ventricular-subventricular zone (V-SVZ). We have previously reported that NSCs from adult V-SVZ are contained in cell populations expressing the carbohydrate SSEA-1/LeX, which exhibit either characteristics of quiescent NSCs (qNSCs) or of actively dividing NSCs (aNSCs) based on the absence or the presence of EGF-receptor, respectively. Using the fluorescence ubiquitination cell cycle indicator-Cdt1 transgenic mice to mark cells in G0/G1phase of the cell cycle, we uncovered a subpopulation of qNSCs which were primed to enter the cell cyclein vitro. Besides, we found that treatment with Syndecan-1, a heparan sulfate proteoglycan involved in NSC proliferation, hastened the division of qNSCs and increased proliferation of aNSCs shortening their G1phasein vitro. Furthermore, administration of Syndecan-1 ameliorated the recovery of neurogenic populations in the V-SVZ after radiation-induced injury providing potential cure for neurogenesis decline during brain aging or after injury.Graphical AbstractDisplay OmittedHighlights•A subpopulation of quiescent NSCs are primed to enter cell cycle•The content of primed quiescent NSCs decreases rapidly with age•Syndecan-1 favors cell cycle progression of NSCsin vitroandin vivoNeuroscience; Stem Cell Research
