摘要：ABSTRACTInduced spawning is more inefficient inClarias magurthan in other fish species such as cyprinids and salmonids. Ovulation can be induced in the femaleC. magurby using pituitary extract or synthetic hormones. However, milt from the maleC. magurcannot be obtained by hand stripping because the volume of seminal fluid in the testes is extremely low. Notably, similar problems are observed in other male catfishes such asC. gariepinusandC. lazera. Because milt from the males cannot be obtained for use in artificial fertilization of eggs, males are invariably sacrificed, and the testicular tissue is excised and macerated to obtain spermatozoa.•We developed an alternative approach that allowed harvesting ofC. magurspermatozoa through surgery for artificial fertilization without sacrificing male fish.•The surgically obtained spermatozoa were used to inseminateC. magureggs; the cross resulted in healthy progeny with a fertilization rate of 80%–98% and hatching of up to 85% of fertilized embryos; similar to those obtained using the conventional sacrificial approach (hatching percentage range of 45%–85%) .•This indicated the viability of partial surgical harvest of testicular tissue in seed production inC. magurfor aquaculture without sacrificing male fish.Graphical AbstractA) Reproductive matureClarias magur(scale bar = 6 cm).B) Surgery ofC. magurunder anaesthetic condition (100 ppm 2-phenoxyethanol) for partial harvest of testicular tissue.C) Collection of spermatozoa from the harvested testicular tissue.D) Cryopreservation of harvested spermatozoa for posterity.E) Artificial insemination ofC. magureggs by freshly harvested spermatozoa.F) Recovery of cryopreserved spermatozoa, quantification, viability assessment and artificial insemination with freshly derived eggs fromC. magurfemale.G) Generation ofC. magurprogeny.Display Omitted
