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  • 标题:Razor scrape assay, an alternative variation to wound and healing assays
  • 本地全文:下载
  • 作者:Jesús Antonio Lara-Reyes ; Mariana Giezi Jimenez-Buendia ; Gonzalo Emiliano Aranda-Abreu
  • 期刊名称:MethodsX
  • 印刷版ISSN:2215-0161
  • 电子版ISSN:2215-0161
  • 出版年度:2020
  • 卷号:7
  • 页码:1-6
  • DOI:10.1016/j.mex.2020.101135
  • 语种:English
  • 出版社:Elsevier
  • 摘要:AbstractCell migration is the process by which cells move through tissues, and it is crucial to carry out a wide variety of physiological and pathological processes. The study methods to evaluate cell migration are very useful tools for biomedical research. Among these methods, the wound and healing assay is one of the simplest, most economical and is widely used in research. However, one of its disadvantages is that the width and shape of the wound can vary among experimental samples since the scraping is carried out manually, representing a difficult variable to control. In the present article a variant of the razor scrape assay is addressed, which eliminates this variation in the width of the wound, thus facilitating the measurement and comparison using the total area of cell migration.•A method that can be carried out under standard culture conditions.•Avoids the disadvantage of variation in width and shape of the wound.•It constitutes a simple, cheap option and multiple advantages over the traditional method.Graphical abstractDisplay Omitted
  • 关键词:Cell culture techniques;Cell migration assays;Cell movement
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