摘要：Abstractcis-Dihydrodiendiols are valuable compounds, finding multiple application as chiral synthons in organic chemistry. The biotechnological route for the generation ofcis-dihydrodiendiols involves the dihydroxylation of aromatic compounds, catalyzed by Rieske non-heme iron dioxygenases. To date, numerous examples of recombinantE. coli, harboring such dioxygenases, can be found in the literature. Nevertheless, there is only a minor number of publications, addressing theE. colicatalyzed degradation ofcis-dihydrodiendiols into catecholsviadehydrogenases. Identification and elimination of such dehydrogenase catalyzed degradation is key for the establishment of enhanced recombinantE. coliplatforms pursuing the production ofcis-dihydrodiendiols. Here, we provide a fast and easy strategy for the identification of promiscuous alcohol dehydrogenases inE. coliBW25113, catalyzing the degradation ofcis-dihydrodiendiols into catechols. This approach is based on the screening of dehydrogenase deficient KEIO strains, regarding their incapability of degrading acis-dihydrodiendiol of choice.•Novel screening strategy forE. coliBW25113 dehydrogenase knock-outs, incapable of degradingcis-dihydrodiendiols was validated forcis-1,2-dihydrocatechol as substrate•Corresponding knock-outs can be used for recombinant production ofcis-dihydrodiendiols•Simple analysis based on liquid chromatography with diode array detector (HPLC-DAD)Graphical abstractDisplay Omitted
关键词：KEIO collection;Screening;HPLC-DAD;Rieske non-heme iron dioxygenase;96 deepwell plate;cis-1,2-dihydrocatechol
