摘要：SummaryThevrille(vri) gene encodes a transcriptional repressor required forDrosophiladevelopment as well as circadian behavior in adults. Alternate first exons producevritranscripts predicted to produce a short VRI isoform during development and long VRI in adults. Avrimutant (vriΔ679) lacking long VRI transcripts is viable, confirming that short VRI is sufficient for developmental functions, yet behavioral rhythms invriΔ679flies persist, showing that short VRI is sufficient for clock output. E-box regulatory elements that drive rhythmic long VRI transcript expression are required for developmental expression of short VRI transcripts. Surprisingly, long VRI transcripts primarily produce short VRI in adults, apparently due to a poor Kozak sequence context, demonstrating that short VRI drives circadian behavior. Thus, E-box-driven long VRI transcripts primarily control circadian rhythms via short VRI, whereas the same E-boxes drive short VRI transcripts that control developmental functions using short VRI.Graphical abstractDisplay OmittedHighlights•vri-E mRNA is sufficient forDrosophiladevelopment and circadian behavior•E-boxes upstream of thevri-ADF promoter are required forDrosophiladevelopment•vri-ADF mRNAs primarily produce short VRI protein rather than long VRI protein•Short VRI protein primarily controlsDrosophiladevelopment and circadian behaviorDevelopmental Genetics; Molecular Biology
