摘要：SummaryDopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-taggedEscherichia colieven when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present inGlioblastoma multiforme. This is crucial becauseGlioblastoma multiformecells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps.Graphical AbstractDisplay OmittedHighlights•Dopamine induces ETs in BV2 microglia and primary adult human microglia•Induced traps are independent of ROS, cell death, and actin polymerization•Microglia ETs are functional and can trapE. coli•Microglia ETs are also present inGlioblastoma multiformeMolecular Biology; Cellular Neuroscience; Immunology; Cell Biology
