摘要：SummaryThe calcium-binding proteins parvalbumin and calbindin are expressed in neuronal populations regulating brain networks involved in spatial navigation, memory processes, and social interactions. Information about the numbers of these neurons across brain regions is required to understand their functional roles but is scarcely available. Employing semi-automated image analysis, we performed brain-wide analysis of immunohistochemically stained parvalbumin and calbindin sections and show that these neurons distribute in complementary patterns across the mouse brain. Parvalbumin neurons dominate in areas related to sensorimotor processing and navigation, whereas calbindin neurons prevail in regions reflecting behavioral states. We also find that parvalbumin neurons distribute according to similar principles in the hippocampal region of the rat and mouse brain. We validated our results against manual counts and evaluated variability of results among researchers. Comparison of our results to previous reports showed that neuron numbers vary, whereas patterns of relative densities and numbers are consistent.Graphical AbstractDisplay OmittedHighlights•Brain-wide, semi-automatic quantification of parvalbumin and calbindin neurons•Largely complementary distribution of calbindin and parvalbumin neurons in mice•Comparison with several previous studies shows variable numbers but similar trends•Similar distribution of parvalbumin neurons in the rat and mouse hippocampal regionHistology; Imaging Anatomy; Optical Imaging; Molecular Neuroscience; Cellular Neuroscience
