摘要：SummaryCD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4+T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32+memory CD4+T cells were enriched in cell-associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32−CD4+fraction. Using multidimensional reduction analysis, several memory CD4+CD32+T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32+CD4+memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32-CD4+T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4+T-cell subsets that contain only a small proportion of the translation-competent reservoir.Graphical abstractDisplay OmittedHighlights•CD32 is rarely expressed in memory CD4+T cells in humanized mice infected with HIV-1•Tissue-resident CD32+CD4+memory T cells are enriched for HIV-1 DNA but not for RNA•CD32+CD4+memory cells are enriched for translation-competent reservoirs•CD32 labels highly activated/exhausted memory T-cell subsets in tissuesMolecular Biology; Immunology ; Virology; Cell Biology
