出版社：Association of College and University Biology Educators
摘要：For the purpose of teaching collegians the fundamentals of biological research, literature explaining the discovery of the gastric proton pump was presented in a 50-min lecture. The presentation included detailed information pertaining to the discovery process. This study was chosen because it demonstrates the importance of having a broad range of knowledge, performing technique with precision, and thinking creatively, in the context of an interesting story about an enzyme which is important in our daily lives. Kasbekar and Durbin were the first team that tried to purify a gastric proton pump for characterization. They isolated the wrong ATPase because of inaccurate technique. Forte et al. improved the technique and isolated the gastric proton pumps. More importantly, for the first time Forte et al. demonstrated that the proton pump is a potassium ion dependent ATPase, or H+,K+- ATPase. The primary literature used here served as a valuable tool to demystify both the process of formulating a testable hypothesis and the pathway for a scientific discovery.
