摘要：SummarySentinel species are playing an indispensable role in monitoring environmental pollution in aquatic ecosystems. Many pollutants found in water prove to be endocrine disrupting chemicals that could cause disruptions in lipid homeostasis in aquatic species. A comprehensive profiling of the lipidome of these species is thus an essential step toward understanding the mechanism of toxicity induced by pollutants. Both the composition and spatial distribution of lipids in freshwater crustaceanGammarus fossarumwere extensively examined herein. The baseline lipidome of gammarids of different sex and reproductive stages was established by high throughput shotgun lipidomics. Spatial lipid mapping by high resolution mass spectrometry imaging led to the discovery of sulfate-based lipids in hepatopancreas and their accumulation in mature oocytes. A diverse and dynamic lipid composition inG. fossarumwas uncovered, which deepens our understanding of the biochemical changes during development and which could serve as a reference for future ecotoxicological studies.Graphical AbstractDisplay OmittedHighlights•Baseline lipidome profiling ofG. fossarumof different sex and reproductive stages•Spatial localization of lipids in gammarid tissue by mass spectrometry imaging•SIMS imaging guided discovery of sulfate-based lipids in hepatopancreas epithelium•Disclosure of a dynamic lipid composition in maturing female oocytesEnvironmental Science; Lipidomics; Metabolomics; Pollution; Zoology
