摘要：The present study aimed to optimize the production of <i>Scenedesmus</i> sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (<b><i>A</i></b>) with a range of 10% to 75% and aeration rate (<b><i>B</i></b>) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day<sup>−1</sup>) was achieved with the 64.26% of (<i><b>A</b></i>) and 3.08 L/min of (<b><i>B</i></b>). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for <i>Scenedesmus</i> sp. Biomass, which has several applications in the pharmaceutical and chemical industry.
