摘要：AbstractBased on the untreated biofilm formation in urinary tract infections (UTIs), current study was focused on the inhibition ofPseudomonas aeruginosa(P. aeruginosa) biofilm formation usingNerium oleander(N. oleander) essential oils. Initially, the LC-MS analysis of antibacterial potential of hydro distillatedN. oleanderessential oils was detected by LC-MS analysis. Initially, the antibacterial nature of essential oils was detected by agar well diffusion method against biofilm formingP. aeruginosa. Then, the possible anti-bacterial fractions of the essential oils were purified by preparative HPLC, and confirmed by analytical HPLC peaks. Possible anti-bacterial components of the fractions were merged together and confirmed by agar well diffusion method. Consecutively, the and minimum biofilm inhibition concentration experiment was proved that the concentration of 200 µg/mL ofpurifiedessential oils was very effective againstP. aeruginosabiofilm formation. The decrease survival rate of the MTT assay result was also supported the minimum biofilm inhibition concentration result. Finally, all the results were more evident; the HPLC purifiedN. oleanderessential oils as a promising anti-biofilm factor againstP. aeruginosabiofilm formation.
