期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2020
卷号：478
期号：1
DOI：10.1088/1755-1315/478/1/012012
语种：English
出版社：IOP Publishing
摘要：Cumulus cells are play a role as nucleus donor in Somatic Cell Nuclear Transfer (SCNT) technology. This study was conducted to find a suitable method for cryopreserving cumulus cells in aim to provide donor cell stock of primary cell culture. The freezing temperature was made at three levels: -20°C, -80 °C, and -196 °C (liquid nitrogen), while cryopreservation was performed by adding cryoprotectants (CPs) that made from 10% Ethylene Glycol (EG) or 10% Dimethyl Sulfoxide (DMSO) in Dulbecco's Phosphate Buffer Saline (DPBS) + 20% New Born Calf Serum (NBCS) medium. The results showed that the freezing temperature, cryoprotectants and their interaction had no significant effects on the cell concentration (p>0.05). The cell viability efficiency was decreased significantly when the cell were freezing at -20°C compared to freezing temperature at -80°C and -196°C (p<0.05). The highest cell viability efficiency was obtained from the cell preserved at -196°C using 10% DMSO. The regression test showed that the decrease in temperature will increase the viability efficiency. In conclusion, cumulus cell primary culture can be cryopreserved in temperature of - 80°C and -196°C with CPs of 10% DMSO or 10%EG.
