标题:Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
摘要:The present study involved in vivo evaluation of the growth promoting effects of thymol and thymol nanoemulsion and their protection against
Salmonella Typhimurium infection in broilers. One-day old 2400 chicks were randomly divided into eight groups; negative and positive control groups fed basal diet without additives and thymol and thymol nanoemulsion groups (0.25, 0.5 and 1% each). At d 23, all chicks except negative control were challenged with
S. Typhimurium. Over the total growing period, birds fed 1% thymol nanoemulsion showed better growth performance even after
S. Typhimurium challenge, which came parallel with upregulation of digestive enzyme genes (
AMY2A,
PNLIP and
CCK). Additionally, higher levels of thymol nanoemulsion upregulated the expression of
MUC-2,
FABP2,
IL-10,
IgA and tight junction proteins genes and downregulated
IL-2 and
IL-6 genes expression. Moreover, 1% thymol nanoemulsion, and to lesser extent 0.5% thymol nanoemulsion and 1% thymol, corrected the histological alterations of cecum and liver postinfection. Finally, supplementation of 1% thymol, 0.5 and 1% thymol nanoemulsion led to increased
Lactobacilli counts and decreased
S. Typhimurium populations and downregulated
invA gene expression postinfection. This first report of supplying thymol nanoemulsion in broiler diets proved that 1% nano-thymol is a potential growth promoting and antibacterial agent.