摘要：SummaryUsingin vivomuscle stem cell (satellite cell)-specific extracellular vesicle (EV) tracking, satellite cell depletion,in vitrocell culture, and single-cell RNA sequencing, we show satellite cells communicate with other cells in skeletal muscle during mechanical overload. Early satellite cell EV communication primes the muscle milieu for proper long-term extracellular matrix (ECM) deposition and is sufficient to support sustained hypertrophy in adult mice, even in the absence of fusion to muscle fibers. Satellite cells modulate chemokine gene expression across cell types within the first few days of loading, and EV delivery of miR-206 to fibrogenic cells repressesWisp1expression required for appropriate ECM remodeling. Late-stage communication from myogenic cells during loading is widespread but may be targeted toward endothelial cells. Satellite cells coordinate adaptation by influencing the phenotype of recipient cells, which extends our understanding of their role in muscle adaptation beyond regeneration and myonuclear donation.Graphical abstractDisplay OmittedHighlights•Widespread myogenic cell communication in muscle during mechanical overload (MOV)•Extracellular vesicles from myogenic progenitors repressWisp1in fibrogenic cells•Fibrogenic fate and chemokines are influenced by satellite cells early during MOV•Prior to fusion, satellite cell communication regulates long-term hypertrophyCell Biology; Functional Aspects of Cell Biology
