摘要：SummaryMicroglia play a key role in brain development, normal homeostasis, and neurodegenerative disorders. Single-cell technologies have led to important findings about microglia, with many animal model studies using single-cell RNA sequencing (scRNA-seq), whereas most human specimen studies using archived frozen brains for single-nucleus RNA sequencing (snRNA-seq). However, microglia compose a small proportion of the total brain tissue; snRNAseq depletes expression of microglia activation genes that characterize many diseases. Here we examine the use of purified, cryopreserved microglia for scRNA-seq. Comparison of scRNA-seq on paired fresh and cryopreserved microglia from rhesus monkeys revealed a high level of correlation of gene expression between the two conditions. Disease-related genes were relatively unaffected, but an increase in immediate-early gene expression was present in cryopreserved cells. Regardless, changes in immediate-early gene expression are still detectable. Cryopreservation of microglia is a suitable procedure for prospectively archiving samples.Graphical abstractDisplay OmittedHighlights•High-quality scRNA-seq can be performed on cryopreserved microglia•Cryopreservation of microglia does not affect disease-related gene expression•Expression of immediate-early genes (IEG) is increased in cryopreserved microglia•scRNA-seq of cyropreserved microglia reveals gene expression changes, including IEGMolecular Neuroscience ; Cellular Neuroscience ; Transcriptomics
