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  • 标题:EF-1α silencing by feeding RNAi suppresses resting cyst formation in Colpoda cucullus Nag-1 strain
  • 本地全文:下载
  • 作者:Y Sogame ; M Hori ; T Matsuoka
  • 期刊名称:Invertebrate Survival Journal
  • 电子版ISSN:1824-307X
  • 出版年度:2016
  • 卷号:13
  • 期号:1
  • 页码:89-93
  • DOI:10.25431/1824-307X/isj.v13i1.89-93
  • 语种:English
  • 出版社:Universita degli Studi di Modena e Reggio Emilia
  • 摘要:It is reported that the expression level of EF-1α in Colpoda cucullus Nag-1 is markedly enhanced within several hours after the onset of encystment induction. In this study, the Colpoda strain (stock EQ-1) known to promptly encyst also showed early EF-1α expression while the strain (stock ES-1) known to show prolonged encystment also showed delayed EF-1α expression. In cells in which EF-1α is silenced by feeding RNAi, the cyst formation was prolonged, but normal cyst walls were formed. These results suggest that Colpoda EF-1α is involved in the early morphogenetic events of the resting cyst formation by accelerating protein translation or cytosleletal dynamics such as microtubule disintegration.
  • 关键词:EF-1α;Colpoda;encystment;feeding RNAi
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