期刊名称：Case Studies in Chemical and Environmental Engineering
印刷版ISSN：2666-0164
出版年度：2021
卷号：3
页码：1-5
DOI：10.1016/j.cscee.2021.100080
语种：English
出版社：Elsevier
摘要：AbstractNovel therapeutic agents discovery have become crucial because humankind is experiencing issues regarding health and the environment. The present study is focusing on thrombolytic agents, which are therapeutics for cardiovascular diseases, eco-friendly, safe and can be used in comparison to synthetic agents that are associated with some side effects and their production is not environment friendly. The main objective of the present research was to evaluate the thrombolytic and cytotoxic potential of different extracts ofEscherchia coli-ATCC35218. Soxhlet extraction method with ethanol was used for extraction from cells. Crude protein was extracted from the cell mass with protein extraction buffer. Crude isoprenoid quinone and polar lipids were extracted by using petroleum ether with methanol and chloroform as extraction solvent. In another method, the extracts were prepared using methanol as well asn-hexane, chloroform, ethyl acetate and water in serial extraction. These organic and aqueous extracts ofE. coliwere dried, weighed, dissolved in DMSO and tested for their hemolytic and thrombolytic potential. Mild to moderate percentage of thrombolytic activity was shown by these extracts and compared with citric acid (standard). Chloroform, ethyl acetate and methanol soluble extracts showed significant thrombolytic activities that were 43.5% ± 0.06, 41.8% ± 0.16 and 40.5% ± 0.07 respectively, while citric acid showed 73.7% ± 0.05. Maximum hemolytic activities were shown by polar lipids, crude methanol extract andn-hexane that are 87.20% ± 0.08, 82.5% ± 0.06 and 73.46%± 0.08 respectively as compared with 0.1% Triton X-100 (positive control) with 94.75% ± 0.15 hemolysis. Finally, results were analyzed statistically through analysis of variance (ANOVA). Further studies on isolation and identification of bioactive thrombolytic agents from each bioactive thrombolytic extract will help us in reporting natural thrombolytic agents fromE.colithat could benefit human medically. This is one of the few reports of thrombolytics available from natural sources.
关键词：KeywordsenEscherchia coliCytotoxic activityThrombolytic activityClot lysisMethanol extract and eco-friendly thrombolytic agent
