期刊名称：Case Studies in Chemical and Environmental Engineering
印刷版ISSN：2666-0164
出版年度：2021
卷号：3
页码：1-6
DOI：10.1016/j.cscee.2021.100084
语种：English
出版社：Elsevier
摘要：AbstractExopolysaccharides (EPS) are the large molecular weight carbohydrate polymers extracted from higher plants, algae, fungi and bacteria. EPS produced by bacteria differ from commercial EPS obtained from plants and algae as they have many unique characteristics. This study was designed to develop hyper EPS-producingBacillus licheniformismutant using different concentrations of ethyl(methanesulfonate) (EMS). The strainB. licheniformisexposed to 50 μg/mL for 120 min of time durations resulted the highest EPS production (2.855 g/L with 87.3% total carbohydrate content). Herein, to make the bioprocess economical, sugarcane molasses was used as a substrate in fermentation medium. Response surface methodology (RSM) was adapted for optimizational purpose and the mutual effect of various parameters influencing EPS yield was studied. At optimized culture conditions i.e., pH7, temperature 37.5 °C, inoculum size 2.5 mL, and 120 h of incubation period, the strainB. licheniformisM4 resulted maximum EPSs production of 9.006 g/L, which was significantly higher than the yield obtained before optimization of fermentation parameters. The improved EPS production byB. licheniformismutant strain, make it a potential candidate for industrial and biotechnological applications.Graphical abstractDisplay OmittedHighlights•Genome engineering ofB. licheniformiswas performed using ethyl(methane sulfonate) mutagen.•Improved EPS yield was obtained by engineered microbe using sugarcane molasses substrate.•RSM optimization resulted more than 4-folds of EPS yield at specified fermentation parameters.
