摘要：SummaryTop-down, context-dependent modulation of visual processing has been a topic of wide interest, including in mouse primary visual cortex (V1). However, the organization of feedback projections to V1 is relatively unknown. Here, we investigated inputs to mouse V1 by injecting retrograde tracers. We developed a software pipeline that maps labeled cell bodies to corresponding brain areas in the Allen Reference Atlas. We identified more than 24 brain areas that provide inputs to V1 and quantified the relative strength of their projections. We also assessed the organization of the projections, based on either the organization of cell bodies in the source area (topography) or the distribution of projections across V1 (bias). Projections from most higher visual and some nonvisual areas to V1 showed both topography and bias. Such organization of feedback projections to V1 suggests that parts of the visual field are differentially modulated by context, which can be ethologically relevant for a navigating animal.Graphical abstractDisplay OmittedHighlights•We built a software pipeline for aligning cell bodies to the standardized brain atlas•Using the pipeline, we quantified brain wide inputs to V1 using retrograde tracers•Projections from higher visual and many nonvisual areas are topographic and biased•Differential feedback inputs may be relevant for visual processing during navigationOptical Imaging; Systems Neuroscience; Sensory Neuroscience; Software
