摘要:Ophthalmic microbial eye membrane is a kind of membrane complex with highly complex structure, but it also has the therapeutic effect of bacteria that can produce microbial eye membrane. Nowadays, there is no effective method to analyze the microbial membrane. Therefore, a quantitative analysis method of ophthalmic microbial membrane function based on microbiological analysis is proposed. The biomass per unit area, substrate coverage and average thickness of the biofilm were quantitatively analyzed with Staphylococcus as material and microbiological analysis method. The structure indexes such as biomass, average thickness and average diffusion distance increased significantly, indicating the transformation process of microbial membrane from occurrence to maturity. Microbiological analysis method can effectively evaluate the occurrence, development and maturation of microbial membrane, and has potential value in studying the theoretical mechanism of microbial membrane formation.