摘要：SummaryDuring chicken skin development, each feather bud exhibits its own polarity, but a population of buds organizes with a collective global orientation. We used embryonic dorsal skin, with buds aligned parallel to the rostral-caudal body axis, to explore whether exogenous electric fields affect feather polarity. Interestingly, brief exogenous current exposure prior to visible bud formation later altered bud orientations. Applying electric pulses perpendicular to the body rostral-caudal axis realigned bud growth in a collective swirl, resembling an electric field pointing toward the anode. Perturbed buds show normal molecular expression and morphogenesis except for their altered orientation. Epithelial-mesenchymal recombination demonstrates the effects of exogenous electric fields are mediated through the epithelium. Small-molecule channel inhibitor screens show Ca2+channels and PI3 Kinase are involved in controlling feather bud polarity. This work reveals the importance of bioelectricity in organ development and regeneration and provides an explant culture platform for experimentation.Graphical abstractDisplay OmittedHighlights•Feather orientation is collectively altered by 3-pulsed electrical stimulations•The electric field topology reshapes polarity cues in the epithelium•Perturbation implicates involvement of Ca2+channels, not planar cell polarity•Molecular characterization shows feather buds are otherwise normalBiological sciences; Cell biology; Developmental biology
