摘要：SummaryLarge-scale mapping of antigens and epitopes is pivotal for developing immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes. Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasiteTrypanosoma cruzienable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show how linear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.Graphical abstractDisplay OmittedHighlights•Genomic shotgun phage display (gPhage) of eukaryotes is feasible and promising.•gPhage allows rapid antigen ID and epitope mapping, including 3D structures.•Conformation epitopes can be identified and validated by using the gPhage platform.•Most Chagas disease antigens are hypothetical proteins rich in repetitive elements.Parasitology; Sequence analysis; Systems biology
