摘要：Despite the fact that Expressed sequence tag EST-SSR markers have become one of the most preferred DNA molecular markers,only a limited number of EST-SSR molecular markers (14 EST-SSRs) is available for the genome of pistachio (Pistacia vera L.).To generate new EST-SSRs for the pistachio genome,1330 pistachio expressed ESTs at the publicly available NCBI database were searched for SSR motifs.The minimum repeat units criteria was ten for mono-nucleotide repeats (MNR),six for dinucleotide repeats (DNR),five for tri-nucleotide repeats (TNR),tetra-nucleotide repeats (TrNR),penta-nucleotide repeats (PNR),and hexa-nucleotide repeats (HNR).A total of 464 SSR motifs spanning 808095 bp were identified in 397 ESTs and primer pairs were designed for 48 SSR motifs.These EST-SSRs will not only increase the EST-SSRs limited availability in pistachio but also facilitate analyses of genetic diversity,population structure,and marker-assisted breeding programs of pistachio (Pistacia vera L.).
