摘要：By using Illumina MiSeq sequencing platform,the microbial population dynamics was quantitatively analyzed during the biofilm cultivation and domestication of lignite upgrading wastewater in a biological contact oxidation reactor.This study aimed to provide a scientific basis for the design and operation of the reaction system.Based on the influent ratio of lignite wastewater,the experiment was divided into three stages: 30%,60% and 100%.The results showed that,after acclimation,the COD removal rate of the biological contact oxidation reactor for lignite upgrading wastewater reached 85.5588.21%,which met the requirements of the subsequent advanced treatment system.Also,with the increase of the proportion of influent lignite wastewater,the population richness and diversity of biofilm microorganism decreased.The high-throughput sequencing experimental outcome showed that Proteobacteria was the dominant community,followed by Bacteroidetes and Firmicutes.However,at the subordinate level,the microbial population structure of biofilm changed,such that,Aquaspirillum showed a gradual disappearance whiles Hydrogenophaga has gradually dominated.
关键词：Lignite upgrading wastewater;Biological contact oxidation;Microbial population structure
