摘要：The freeze-tolerant Rana chensinensis (R.chensinensis),is one of few vertebrate organisms that can tolerate freezing.Physiologically,R.chensinensis show no signs of muscle movement,breathing,heartbeat,or brain activity for several weeks or months at a time but emerge unharmed upon thawing.Given that R.chensinensis cannot receive polyunsaturated fatty acids (PUFAs) from the environment during freezing,the involvement of the level of PUFAs plays a very important role in maintaining cell structure,membrane fluidity and enzyme activity in response to freezing is of interest.Ovi-ductus Ranae is the fallopian tube of R.chensinensis female frog,and is the main medicinal part of R.chensinensis.Because it is not easy to find the R.chensinensis during the deep hibernation period,we collected R.chensinensis in five periods from awakening period to diaspora hibernation.Two years of R.chensinensis were collected,and it was found that the content of PUFAs in Oviductus Ranae gradually increased and reached the highest during the diaspora hibernation period.AAH73571.1 is a key enzyme gene for fatty acid desaturase (FAD) in Ovi-ductus Ranae.It’s expression results were consistent with the results of PUFAs content,showing a gradual increase pattern,and also reached the highest in the diaspora hibernation period.The change law of the PUFAs content in Oviductus Ranae at different growth stages of R.chensinensis was clarified.It was confirmed that during the hibernation of R.chensinensis,in order to ensure that it can survive the hibernation period,the expression of key FAD enzyme genes in Oviductus Ranae increased,thereby increasing the amount of PUFAs synthesis.The cold-resistance mechanism of R.chensinensis was explained from the regularity of PUFAs changes in Oviductus Ranae.
