摘要：Applicability of Bacillus and Trichoderma isolates,CuSo4,Maxim Fs and bury of sclerotia as physical treatment towards the supreme isolate (Scu) of Sclerotium cepivorum causing onion white rot disease was tested.Mycelium of the pathogen was totally inhibited due to use 1.0 g L-1 of the fungicide Maxim Fs.Based on their antagonistic,biochemical and molecular aspects,B1o and T2 isolates were identified as B.nakamurai and T.asperellum though 16S rRNA and 18S rDNA genomic sequencing tests,respectively.Under naturally infested field conditions,bury of sclerotia at 20 Cm depth from soil surface was the superior treatment,by which no disease incidence or severity during 2016 and 2017 seasons were recorded and disease reduction reached its fulfilled (100 %).Results showed higher magnitudes of peroxidase than polyphenoloxidase activeties due to all treatments compared to control,with pronounced induction by Maxim Fs.For sclerotial depth and biological treatments,low enzyme activities were obtained.Higher magnitudes of conjugated,free and total phenolic compounds were then obtained due to T.asperellum during both seasons.Average of total phenolic content reached its maximum of 10.20 mg / g fresh weights by T.asperellum,indicating large accumulated in onion cells due to non-enzymatic oxidation of phenolic compounds.
关键词：Antagonism;peroxidase;polyphenoloxidase;genomic sequences;onion white rot disease.
