摘要：The aim of this study is to sterilized nodal explants of 13 Peach germplasm (Early green,Flame crust,Spring crust,Florida king,Early grand,669 No.2.,No.2,A7,Chaki pakora,A4,4.5,Clark king,and A2) from diverse agro-ecological zones of Pakistan,so as to eliminate microbial contamination and to increase survival percentage.The ex-plants were treated with different concentrations of ethyl alcohol,sodium hypochlorite and with double distilled water for different time periods in Murashige and Skoog (MS) media supplemented with BAP (1mg/l).Results showed that Ethanol 70% (1min) + Sodium hypochlorite 28% (15min) gave 100% survival in genotypes Early green,669 No.2,A7,4.5,and Clark king as compared to control,The fungal contamination was noted in Florida king (80%),Chaki pakora (60%),and Early green (20%) while,bacterial contamination was found in 669 No.2 (100%),Flame crust (20%) and viral contamination was found in Clark king (20%) when disinfected with 100% double distilled water.The current work proved that 100 % double distilled water was not enough for surface sterilization of Peach ex-plants as fungal,bacterial,viral and combination of other potent sterilization agents are required.
