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  • 标题:COLLECTION OF,AND SEROLOGICAL AND MOLECULAR VIRAL DISEASE DETECTION IN LOCAL GARLIC (ALLIUM SATIVUM L.) ACCESSIONS COLLECTED FROM NORTHERN CYPRUS
  • 本地全文:下载
  • 作者:Nihat Yilmaz ; Hakan Fidan
  • 期刊名称:Fresenius Environmental Bulletin
  • 印刷版ISSN:1018-4619
  • 出版年度:2021
  • 卷号:30
  • 期号:1
  • 页码:569-576
  • 语种:English
  • 出版社:PSP Publishing
  • 摘要:This study was conducted by collecting sam-ples of garlic (Allizum sativum L.) and studying itsmorphological characteristics to determine which vi-ral diseases are causing an economic loss of yield incultivation areas in Northern Cyprus.Consideringtheir morphological characteristics,87 garlic geno-types were collected from 6 different locations inNorthern Cyprus,83 of which were infected withone or more viruses.The isolates obtained weretested against Garlic common latent virus (Gar-CLV),Leek yellow strip virus (LYSV),Shallot la-tent virus (SLV),Cucumber mosaic virus (CMV),Tobacco mosaic virus (TMV),and Onion yellowdwarf virus (OYDV).For diagnoses,both double an-tibody sahdwich enzyme-linked immunosorbent as-say and reverse transcription poly merase chain reac-tion techniques were used.Eighty-seven garlic-leafsamples and 87 garlic clove samples were tested.The results from these samples were similar .Amongthe 87 plants tested,38 (44%) were positive for Gar-CLV,25 (29%) for LYsv,15 (17%) for SLV,and 5(5.7%) for OYDV .GarCLV was found to be themost common virus in the isolates.
  • 关键词:Garlic (Allium sativum L.);Garlic common latent virus;Onion yellow dwarf virus;Leek yellow stripe vinus;RT-PCR technique.
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